Effect of recombinant human interleukin 2 on neutrophil adherence to endothelial cells in vitro
Identifieur interne : 003E51 ( Main/Exploration ); précédent : 003E50; suivant : 003E52Effect of recombinant human interleukin 2 on neutrophil adherence to endothelial cells in vitro
Auteurs : Jun Li [Canada] ; Steve Gyorffy [Canada] ; Sharon Lee [Canada] ; Cheuk S. Kwok [Canada]Source :
- Inflammation [ 0360-3997 ] ; 1996-08-01.
Abstract
Abstract: Human neutrophils were demonstrated to possess interleukin-2 receptor (IL-2R)β andγ chains, notα chain and the binding of IL-2 to the IL-2Rβ chain on neutrophils plays an important regulatory role in neutrophil functions. We have investigated in this study the hypothesis that recombinant human IL-2(rhIL-2) can directly activate human neutrophils and increase their adherence to human umbilical vein endothelial cells (HUVEC). In an in vitro microtiter adherence assay, rhIL-2 significantly stimulated neutrophil adherence to HUVEC in a dose- and time-dependent manner. rhILI-2 concentration at 2000 u/ml and 2 hour incubation gave the best neutrophil stimulation. Treatment of neutrophils with rhIL-2 increased the expression of adhesion molecule CD18. Pretreatment of the stimulated neutrophils with a blocking monoclonal antibody to CD18 decreased but not completely blocked the adherence of neutrophils to HUVEC. These data suggest that rhIL-2 can directly stimulate and increase neutrophil adherence to HUVEC by enhancing the expression of CD18 and possibly other adhesion molecules on neutrophil surface. This may be a critical step in the early stage of the vascular leak syndrome (VLS) associated with high dose IL-2 therapy.
Url:
DOI: 10.1007/BF01486739
Affiliations:
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Kwok</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:92C19ACAD638056E63FF41C7B18DF591C72EAA1E</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1007/BF01486739</idno><idno type="url">https://api-v5.istex.fr/document/92C19ACAD638056E63FF41C7B18DF591C72EAA1E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001B32</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001B32</idno><idno type="wicri:Area/Istex/Curation">001B32</idno><idno type="wicri:Area/Istex/Checkpoint">001861</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001861</idno><idno type="wicri:doubleKey">0360-3997:1996:Li J:effect:of:recombinant</idno><idno type="wicri:Area/Main/Merge">004541</idno><idno type="wicri:Area/Main/Curation">003E51</idno><idno type="wicri:Area/Main/Exploration">003E51</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Effect of recombinant human interleukin 2 on neutrophil adherence to endothelial cells in vitro</title><author><name sortKey="Li, Jun" sort="Li, Jun" uniqKey="Li J" first="Jun" last="Li">Jun Li</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Physics, Hamilton Regional Cancer Center, 699 Concession Street, L8V 5C2, Hamilton, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name sortKey="Gyorffy, Steve" sort="Gyorffy, Steve" uniqKey="Gyorffy S" first="Steve" last="Gyorffy">Steve Gyorffy</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Physics, Hamilton Regional Cancer Center, 699 Concession Street, L8V 5C2, Hamilton, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sharon" sort="Lee, Sharon" uniqKey="Lee S" first="Sharon" last="Lee">Sharon Lee</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Physics, Hamilton Regional Cancer Center, 699 Concession Street, L8V 5C2, Hamilton, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name sortKey="Kwok, Cheuk S" sort="Kwok, Cheuk S" uniqKey="Kwok C" first="Cheuk S." last="Kwok">Cheuk S. Kwok</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Physics, Hamilton Regional Cancer Center, 699 Concession Street, L8V 5C2, Hamilton, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Inflammation</title><title level="j" type="abbrev">Inflammation</title><idno type="ISSN">0360-3997</idno><idno type="eISSN">1573-2576</idno><imprint><publisher>Kluwer Academic Publishers-Plenum Publishers</publisher><pubPlace>New York</pubPlace><date type="published" when="1996-08-01">1996-08-01</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="361">361</biblScope><biblScope unit="page" to="372">372</biblScope></imprint><idno type="ISSN">0360-3997</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0360-3997</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Human neutrophils were demonstrated to possess interleukin-2 receptor (IL-2R)β andγ chains, notα chain and the binding of IL-2 to the IL-2Rβ chain on neutrophils plays an important regulatory role in neutrophil functions. We have investigated in this study the hypothesis that recombinant human IL-2(rhIL-2) can directly activate human neutrophils and increase their adherence to human umbilical vein endothelial cells (HUVEC). In an in vitro microtiter adherence assay, rhIL-2 significantly stimulated neutrophil adherence to HUVEC in a dose- and time-dependent manner. rhILI-2 concentration at 2000 u/ml and 2 hour incubation gave the best neutrophil stimulation. Treatment of neutrophils with rhIL-2 increased the expression of adhesion molecule CD18. Pretreatment of the stimulated neutrophils with a blocking monoclonal antibody to CD18 decreased but not completely blocked the adherence of neutrophils to HUVEC. These data suggest that rhIL-2 can directly stimulate and increase neutrophil adherence to HUVEC by enhancing the expression of CD18 and possibly other adhesion molecules on neutrophil surface. This may be a critical step in the early stage of the vascular leak syndrome (VLS) associated with high dose IL-2 therapy.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Li, Jun" sort="Li, Jun" uniqKey="Li J" first="Jun" last="Li">Jun Li</name></noRegion><name sortKey="Gyorffy, Steve" sort="Gyorffy, Steve" uniqKey="Gyorffy S" first="Steve" last="Gyorffy">Steve Gyorffy</name><name sortKey="Kwok, Cheuk S" sort="Kwok, Cheuk S" uniqKey="Kwok C" first="Cheuk S." last="Kwok">Cheuk S. Kwok</name><name sortKey="Lee, Sharon" sort="Lee, Sharon" uniqKey="Lee S" first="Sharon" last="Lee">Sharon Lee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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